![Can anyone explain why do we get diffused bands in western blot? and any trouble shooting techniques? Can anyone explain why do we get diffused bands in western blot? and any trouble shooting techniques?](https://www.researchgate.net/profile/Sv-Mg-2/post/Can-anyone-explain-why-do-we-get-diffused-bands-in-western-blot-and-any-trouble-shooting-techniques/attachment/5dcbef47cfe4a777d4f05210/AS%3A824745348059138%401573646022659/image/1.png)
Can anyone explain why do we get diffused bands in western blot? and any trouble shooting techniques?
![A bead-based western for high-throughput cellular signal transduction analyses | Nature Communications A bead-based western for high-throughput cellular signal transduction analyses | Nature Communications](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fncomms12852/MediaObjects/41467_2016_Article_BFncomms12852_Fig1_HTML.jpg)
A bead-based western for high-throughput cellular signal transduction analyses | Nature Communications
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Frontiers | Non-specific Detection of a Major Western Blotting Band in Human Brain Homogenates by a Multitude of Amyloid Precursor Protein Antibodies | Aging Neuroscience
![Troubleshooting tips for western blotting | Troubleshooting Tips | Support | Signalway Antibody | Signalway Antibody Troubleshooting tips for western blotting | Troubleshooting Tips | Support | Signalway Antibody | Signalway Antibody](https://www.sabbiotech.com/images/upload/Image/image015.gif)